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Application Data
- Human IL-8 ELISA -
Feature
Quantity of IL-8 contained in sample can be determined with Sandwich ELISA method. Addition of chromogenic hydrogen donor TMB into enzyme reaction enables absorptiometric colorimetry. TMB is offered commercially in form of solution which does not need any trimming as material for making up enzyme substrate.
Application
It is implied that IL-8 is connected with noneffective inflammatory disorder. IL-8 is used for inspection of rheumatic gout, gout, scabies, ARD-Adult Respiratory Distress Syndrome, asthma and so on.
Detection limit : 0.027ng/ml (2SD)
Linearity range : 0.02~1.00ng/ml
Instrument : MTP-650FA Multimode Microplate reader
Reagent :
Labeling Enzyme : HR
Substrate solution : TMB-ONE (Fermentas #R0761)
TMB : More sensitive than O-phenylenediamine/Stable sensitivity/Non-toxic


Condition of measurement
Ex/λ:450nm
Flash times: 20times
MODE1: Abs
Direction: Vertical
Mode: END
TEMP: OFF
Solid phasing antibody
V
Blocking
V
Antigen reaction 2h
V
Primary Antibody 1h
V
Enzyme probed secondary antibody 1h
V
Substrate response 20min
V
Absorption reaction

Data: Provided by HHT BMC



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